Fanucci Group

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

C.P. Easterling, T. Kubo, Z.M. Orr, G.E. Fanucci and B.S. Sumerlin "Synthetic upcycling of polyacrylates through organocatalyzed post-polymerization modification"Chem. Sci,2017.

Z. Liu, X. Huang, L. Hu, L. Pham, K.M. Poole, Y Tang, B.P. Mahon, W. Tang, K. Li, N.E. Goldfarb, B.M. Dunn, R. McKenna, G.E. Fanucci "Effects of hinge-region natural polymorphisms on human immunodeficiency virus-type 1 protease structure, dynamics, and drug pressure evolution"Journal of Biological Chemistry, 291(43), 22741-227562016.

J.M. Esquiaqui, E.M. Sherman, J.D. Ye, and G.E. Fanucci "Conformational Flexibility and Dynamics of the Internal Loop and Helical Regions of the Kink-Turn Motif in the Glycine Riboswitch by Site-Directed Spin-Labeling" Biochemistry 55 (31), 4295-4305, 2016.

A.N. Smith, U.T. Twahir, T. Dubroca and G.E. Fanucci, and J.R. Long "Molecular Rationale for Improved Dynamic Nuclear Polarization of Biomembranes"The Journal of Physical Chemistry B 120 (32), 7880-7888,2016.

L. Song, Z. Liu, P. Kaur, J.M. Esquiaqui, R.I. Hunter, S.Hill, G. Smith and G.E. Fanucci "Toward increased concentration sensitivity for continuous wave EPR investigations of spin-labeled biological macromolecules at high fields"PJournal of Magnetic Resonance, 265, 188-196,2016.

Z. Liu, T.M. Casey, M.E. Blackburn, X. Huang, L. Pham, I. M. S. De Vera, J.D. Carter, J.L. Kear, A.M. Veloro, L. Galiano, G.E. Fanucci "Pulsed EPR characterization of HIV-1 protease conformational sampling and inhibitor-induced population shifts"Phys. Chem. Chem. Phys., 18(8), 5819-5831,2016.

T. M. Casey and G.E. Fanucci "Spin labeling and Double Electron-Electron Resonance (DEER) to Deconstruct Conformational Ensembles of HIV Protease" Methods in Enzymology Vol. 564, pp. 153-187,2015.

A.N. Smith, M.A. Caporini, G.E. Fanucci, and J.R. Long, "A Method for Dynamic Nuclear Polarization Enhancement of Membrane Proteins" Angew. Chem. Int. Ed., 54: 1542-1546,2015.

J.D. Carter, J.D. Mathias, E.F. Gomez, Y. Ran, F. Xu, L. Galiano, N.Q. Tran, P.W. D'Amore, C.S. Wright, D.K. Chakravorty, and G.E. Fanucci "Characterizing Solution Surface Loop Conformational Flexibility of the GM2 Activator Protein"The Journal of Physical Chemistry B 118 (36), 10607-106172014.

J.M. Esquiaqui, E.M. Sherman, J.D. Ye and G.E. Fanucci "Site-directed spin-labeling strategies and electron paramagnetic resonance spectroscopy for large riboswitches".Methods in Enzymology, 549(C), 287-311,2014.

T.M. Casey, Z. Liu, J.M. Esquiaqui, N.L. Pirman, E. Milshteyn and G.E. Fanucci "Continuous wave W- and D-Band EPR spectroscopy offer "sweet- spots" for characterizing conformational changes and dynamics in intrinsically disordered proteins" Biochemical and Biophysical Research Communications, 450(1), 723-7282014.

J.D. Carter, E.G. Gonzales, X. Huang, A.N. Smith, I.M.S. De Vera, P.W..D'Amore, J.R. Rocca, M.M. Goodenow, B.M. Dunn, G.E. Fanucci "Effects of PRE and POST therapy drug-pressure selected mutations on HIV-1 protease conformational sampling" FEBS Letters, 588,2014.

A.L. Turner, O. Braide, F.D. Mills, G.E. Fanucci, J.R. Long, "Residue specific partitioning of KL4 into phospholipid bilayers"Biochimica et Biophysica Acta - Biomembranes, 1838(12), 3212-3219, 2014.

J.M. Esquiaqui, E.M. Sherman, S.A. Ionescu, Y. Jing-Dong and G.E. Fanucci "Characterizing the Dynamics of the Leader-Linker Interaction in the Glycine Riboswitch with Site-Directed Spin Labeling"Biochemistry 2014 53 (22), 3526-3528, 2014.

X. Huang, I.M.S. De Vera, A.M. Veloro, J.R. Rocca, C. Simmerling, B.M. Dunn, and G.E. Fanucci "Backbone1H,13C, and15N chemical shift assignment for HIV-1 protease subtypes and multi-drug resistant variant MDR 769"Biomolecular NMR Assignments, 7(2), 199-202,2013.

I.M.S. De Vera, M.E. Blackburn, L. Galiano, and G.E. Fanucci "Pulsed EPR distance measurements in soluble proteins by Site-Directed Spin Labeling (SDSL)"Current Protocols in Protein Science, (SUPPL.74),2013.

I.M.S. De Vera, A.N. Smith, M.C.A Dancel, X. Huang, B.M. Dunn, and G.E. Fanucci "Elucidating a Relationship between Conformational Sampling and Drug Resistance in HIV-1 Protease"Biochemistry 52 (19), 3278-3288,2013.

S. Uthandi, L. Prunetti, I.M.S. De Vera, G.E. Fanucci, A. Angerhofer, and J.A. Maupin-Furlow, "Enhanced archaeal laccase production in recombinant Escherichia coli by modification of N-terminal propeptide and twin arginine translocation motifs"Journal of Industrial Microbiology and Biotechnology, 39(10), 1523-1532,2012.

X. Huang, I.M.S. De Vera, A.M. Veloro, M.E. Blackburn, J.L. Kear, J.D. Carter, J.R. Rocca, C. Simmerling, B.M. Dunn, and G.E. Fanucci "Inhibitor-Induced Conformational Shifts and Ligand-Exchange Dynamics for HIV-1 Protease Measured by Pulsed EPR and NMR Spectroscopy"The Journal of Physical Chemistry B 116 (49), 14235-14244,2012.

N.L. Pirman, E. Milshteyn, L. Galiano, J.C. Hewlett and G.E. Fanucci, "Characterization of the disordered-to-?-helical transition of IA3 by SDSL-EPR spectroscopy"Protein Science, 20: 150-159,2011.

I.M.S. De Vera, M.E. Blackburn, and G.E. Fanucci "Correlating Conformational Shift Induction with Altered Inhibitor Potency in a Multidrug Resistant HIV-1 Protease Variant"Biochemistry 51 (40), 7813-7815,2012.

N.L. Hepowit, S. Uthandi, H.V. Miranda, M. Toniutti, L. Prunetti, O. Olivarez, I.M.S. De Vera, G.E. Fanucci, S. Chen, and J.A. Maupin-Furlow, "Archaeal JAB1/MPN/MOV34 metalloenzyme (HvJAMM1) cleaves ubiquitin-like small archaeal modifier proteins(SAMPs) from protein-conjugates."Molecular Microbiology, 86: 971-987,2012.

X. Huang, M.D. Britto, J.L. Kear-Scott, C.D. Boone, J.R. Rocca, C. Simmerling, R. McKenna, M. Bieri, P. Gooley, B.M. Dunn, and G.E. Fanucci, "The role of select subtype polymorphisms on HIV-1 protease conformational sampling and dynamics"Journal of Biological Chemistry., 289(24), 17203-17214,2014.

R.S. Farver, F. D. Mills, V.C. Antharam, J. N. Chebukati, G.E. Fanucci, J.R. Long "Lipid Polymorphism Induced by Surfactant Peptide SP-B1.25"Biophysical Journal, 99(6), 1773-1782,2010.

T.E. Frederick, P.C. Goff, C.E. Mair, R.S. Farver, J.R., Long and Fanucci and G. E. Fanucci "Effects of the endosomal lipid bis(monoacylglycero)phosphate on the thermotropic properties of DPPC: A2H NMR and spin label EPR study"Chemistry and Physics of Lipids, 163(7), 703-711,2010.

J.D. Tipton, J.D. Carter, J.D. Mathias, M.R. Emmett, G.E. Fanucci, A.G. Marshall "Sequential Proteolysis and High-Field FTICR MS To Determine Disulfide Connectivity and 4-Maleimide TEMPO Spin-Label Location in L126C GM2 Activator Protein"Analytical Chemistry 81 (18), 7611-7617,2009.

J.D. Mathias, Y. Ran, J.D. Carter, and G.E. Fanucci "Interactions of the GM2 activator protein with phosphatidylcholine bilayers: A site-directed spin-labeling power saturation study"Biophysical Journal, 97(5), 1436-1444,2009.

Y. Ran and G.E Fanucci "Ligand extraction properties of the GM2 activator protein and its interactions with lipid vesicles" Journal of the American Chemical Society 131 (41), 14650-14651,2009.

M.E Blackburn, A.M. Veloro, and G.E. Fanucci, "Monitoring Inhibitor-Induced Conformational Population Shifts in HIV-1 Protease by Pulsed EPR Spectroscopy" Biochemistry 48 (37), 8765-8767,2009.

J. L. Kear, M.E. Blackburn, A.M. Veloro, B.M. Dunn, and G.E. Fanucci, "Subtype Polymorphisms Among HIV-1 Protease Variants Confer Altered Flap Conformations and Flexibility" Journal of the American Chemical Society 131 (41), 14650-14651,2009.

T.E. Frederick, J.N. Chebukati, C.E. Mair, P.C. Goff and G.E. Fanucci, “Bis(monoacylglycero)phosphate Forms Stable Small Lamellar Vesicle Structures: Insights into Vesicular Body Formation in Endosomes” Biophys. J., accepted, 2009.

L. Galiano, M.E. Blackburn, A.M. Veloro, M. Bonora, and G.E. Fanucci, “Solute Effects on Spin Labels at an Aqueous Exposed Site in the Flap Region of HIV-1 Protease” J. Phys. Chem. B, ASAP 2009.

L. Galiano, D. Fengu, A.M. Veloro, M.E. Blackburn, C. Simmerling and G.E. Fanucci, “Drug Pressure Induced Mutations in HIV-1 Protease alter Flap Conformations” J. Am. Chem. Soc. ASAP 2008.

Y. Ran and G.E. Fanucci “A Dansyl-Fluorescence Based Assay for Monitoring Kinetics of Lipid Extraction and Transfer” Notes and Tips Communication, Anal. Biochem. ,382(2), 132-4, 2008.

L. Galiano, M. Bonora, G. E. Fanucci “Interflap Distances in HIV-1 Protease Determined by Pulsed EPR Measurements” J. Am. Chem. Soc., 129, 11004-5 2007.

V. Lukova, M. Peng, G. E. Fanucci, R. Tandlich, A. Hinderliter, B. Maity, E.Manivannan, G. R. Cook, S. Balaz. "Drug-Membrane Interactions Studied in Phospholipid Monolayers Adsorbed on Non-Porous Aklylated Microspheres" J. Biomolecular Screening, 12, 186-202, 2007.

M. Kim, G.E. Fanucci and D.S. Cafiso “Substrate-Dependent Signaling in TonB-Dependent Transporters Investigated Using Site-Directed Spin Labeling” Proc. Nat. Acad. Sci.,104, 11975-80, 2007.

G. E. Fanucci and D. S. Cafiso "Recent Advances and Applications in Site-Directed Spin Labeling" Curr Opin Struc Bio, 16, 644-53, 2006.

M. Kim, Q. Xu, G. E. Fanucci, D. S. Cafiso "Solutes Modify a Conformational Transition in a Membrane Transport Protein" Biophysical Journal, 90, 2922-9, 2006.

G. E. Fanucci, J. Y. Lee, D. S. Cafiso “Membrane Mimetic Environments Alter the Conformation of the Outer Membrane Protein BtuB” J. Am. Chem. Soc., 125, 13932-13933, 2003.

G. E. Fanucci, J. Y. Lee, D. S. Cafiso “Spectroscopic Evidence that Osmolytes Used in Crystallization Buffers Inhibit a Conformational Change in a Membrane Protein” Biochemistry, 42, 13106-13112, 2003.

 G. E. Fanucci, N. Cadieux, R. J. Kadner, D. S. Cafiso “Competing Ligands Stabilize Alternate Conformations of the Energy Coupling Motif of a TonB-Dependent Outer Membrane Transporter” Proc. Nat. Acad. Sci., 100, 11382-11387, 2003.

 G. E. Fanucci, K. A. Coggshall, N. Cadieux, M Kim, R. J. Kander, D. S. Cafiso “Substrate-Induced Conformational Changes of the Periplasmic N-terminus of an Outer-Membrane Transporter by Site-Directed Spin Labeling” Biochemistry, 42, 1391-1400, 2003.

 G. E. Fanucci, N. Cadieux, C. A. Piedmont, R. J. Kander, D. S. Cafiso “Structure and Dynamics of the b-barrel of the Membrane Transporter, BtuB by Site-Directed Spin Labeling” Biochemistry, 41, 11543-11551, 2002.

A. A. Frazier, M. A. Wisner, N. J. Malmberg, K. G. Victor, G. E. Fanucci, E. A. Nalefski, J. J. Falke, D. S. Cafiso. “Membrane Orientation and Position of the C2 Domain from cPLA2 by Site-Directed Spin Labeling” Biochemistry, 41, 6282-6292, 2002.

G. E. Fanucci, R. Backov, R. Fu, D. R. Talham “Multiple Bilayer Dipalmitoylphosphatidylserine (DPPS) LB Films Stabilized with Transition Metal Ions.” Langmuir, 17, 1660, 2001.